Part:BBa_K137069:Design

Pyruvate oxidase (SpxB)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1653
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Removal of a PstI site very near the 5' end with PCR primers.

Source

Genomic PCR from a prepared genome of Streptococcus pneumoniae R6 from ATCC (# BAA-255D-5)